Membrane:Article Title: mRNA vaccine encoding Gn Provides Protection Against Severe Fever with Thrombocytopenia Syndrome Virus in mice
Article Snippet: .. The membrane was then incubated overnight at 4°C with an anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41156; Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700S; Cell Signaling Technology, Danvers, MA, USA). .. Secondary antibodies used were anti-rabbit or anti-mouse antibodies conjugated with horseradish peroxidase (1:5000 dilution; A120-101P or A90-116P; BETHYL, Montgomery, TX, USA).
Article Title: mRNA vaccine encoding Gn provides protection against severe fever with thrombocytopenia syndrome virus in mice.
Article Snippet: .. The membrane was then incubated overnight at 4 °C with an anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41156; Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700 S; Cell Signaling Technology, Danvers, MA, USA). .. The secondary antibodies used were antirabbit or anti-mouse antibodies conjugated with horseradish peroxidase (1:5000 dilution; A120-101P or A90-116P; BETHYL, Montgomery, TX, USA).
Article Title: Heterologous vaccination utilizing viral vector and protein platforms confers complete protection against SFTSV.
Article Snippet: .. After blocking, the membrane was incubated overnight at 4°C with anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41,156, Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700S, Cell Signaling Technology, Danvers, MA, USA). .. Anti-rabbit or anti-mouse antibody conjugated with horse radish peroxidase (1:5000 dilution, A120-101P or A90-116P, BETHYL, Montgomery, TX, USA) was used as the secondary antibody.
Article Title: Heterologous vaccination utilizing viral vector and protein platforms confers complete protection against SFTSV
Article Snippet: .. After blocking, the membrane was incubated overnight at 4°C with anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41,156, Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700S, Cell Signaling Technology, Danvers, MA, USA). .. Anti-rabbit or anti-mouse antibody conjugated with horse radish peroxidase (1:5000 dilution, A120-101P or A90-116P, BETHYL, Montgomery, TX, USA) was used as the secondary antibody.
Article Title: mRNA vaccine encoding Gn provides protection against severe fever with thrombocytopenia syndrome virus in mice
Article Snippet: .. The membrane was then incubated overnight at 4 °C with an anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41156; Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700 S; Cell Signaling Technology, Danvers, MA, USA). .. The secondary antibodies used were anti-rabbit or anti-mouse antibodies conjugated with horseradish peroxidase (1:5000 dilution; A120-101P or A90-116P; BETHYL, Montgomery, TX, USA).
Article Title: Heterologous prime-boost regimen elicits potent humoral and cell-mediated immune responses and provides complete protection against SFTSV
Article Snippet: .. After blocking, the membrane was incubated overnight at 4°C with anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41156, Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700S, Cell Signaling Technology, Danvers, MA, USA). .. Anti-rabbit or anti-mouse antibody conjugated with horse radish peroxidase (1:5000 dilution, A120-101P or A90-116P, BETHYL, Montgomery, TX, USA) was used as the secondary antibody.
Incubation:Article Title: mRNA vaccine encoding Gn Provides Protection Against Severe Fever with Thrombocytopenia Syndrome Virus in mice
Article Snippet: .. The membrane was then incubated overnight at 4°C with an anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41156; Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700S; Cell Signaling Technology, Danvers, MA, USA). .. Secondary antibodies used were anti-rabbit or anti-mouse antibodies conjugated with horseradish peroxidase (1:5000 dilution; A120-101P or A90-116P; BETHYL, Montgomery, TX, USA).
Article Title: mRNA vaccine encoding Gn provides protection against severe fever with thrombocytopenia syndrome virus in mice.
Article Snippet: .. The membrane was then incubated overnight at 4 °C with an anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41156; Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700 S; Cell Signaling Technology, Danvers, MA, USA). .. The secondary antibodies used were antirabbit or anti-mouse antibodies conjugated with horseradish peroxidase (1:5000 dilution; A120-101P or A90-116P; BETHYL, Montgomery, TX, USA).
Article Title: Heterologous vaccination utilizing viral vector and protein platforms confers complete protection against SFTSV.
Article Snippet: .. After blocking, the membrane was incubated overnight at 4°C with anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41,156, Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700S, Cell Signaling Technology, Danvers, MA, USA). .. Anti-rabbit or anti-mouse antibody conjugated with horse radish peroxidase (1:5000 dilution, A120-101P or A90-116P, BETHYL, Montgomery, TX, USA) was used as the secondary antibody.
Article Title: Heterologous vaccination utilizing viral vector and protein platforms confers complete protection against SFTSV
Article Snippet: .. After blocking, the membrane was incubated overnight at 4°C with anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41,156, Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700S, Cell Signaling Technology, Danvers, MA, USA). .. Anti-rabbit or anti-mouse antibody conjugated with horse radish peroxidase (1:5000 dilution, A120-101P or A90-116P, BETHYL, Montgomery, TX, USA) was used as the secondary antibody.
Article Title: mRNA vaccine encoding Gn provides protection against severe fever with thrombocytopenia syndrome virus in mice
Article Snippet: .. The membrane was then incubated overnight at 4 °C with an anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41156; Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700 S; Cell Signaling Technology, Danvers, MA, USA). .. The secondary antibodies used were anti-rabbit or anti-mouse antibodies conjugated with horseradish peroxidase (1:5000 dilution; A120-101P or A90-116P; BETHYL, Montgomery, TX, USA).
Article Title: Heterologous prime-boost regimen elicits potent humoral and cell-mediated immune responses and provides complete protection against SFTSV
Article Snippet: .. After blocking, the membrane was incubated overnight at 4°C with anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41156, Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700S, Cell Signaling Technology, Danvers, MA, USA). .. Anti-rabbit or anti-mouse antibody conjugated with horse radish peroxidase (1:5000 dilution, A120-101P or A90-116P, BETHYL, Montgomery, TX, USA) was used as the secondary antibody.
Blocking Assay:Article Title: Heterologous vaccination utilizing viral vector and protein platforms confers complete protection against SFTSV.
Article Snippet: .. After blocking, the membrane was incubated overnight at 4°C with anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41,156, Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700S, Cell Signaling Technology, Danvers, MA, USA). .. Anti-rabbit or anti-mouse antibody conjugated with horse radish peroxidase (1:5000 dilution, A120-101P or A90-116P, BETHYL, Montgomery, TX, USA) was used as the secondary antibody.
Article Title: Heterologous vaccination utilizing viral vector and protein platforms confers complete protection against SFTSV
Article Snippet: .. After blocking, the membrane was incubated overnight at 4°C with anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41,156, Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700S, Cell Signaling Technology, Danvers, MA, USA). .. Anti-rabbit or anti-mouse antibody conjugated with horse radish peroxidase (1:5000 dilution, A120-101P or A90-116P, BETHYL, Montgomery, TX, USA) was used as the secondary antibody.
Article Title: Heterologous prime-boost regimen elicits potent humoral and cell-mediated immune responses and provides complete protection against SFTSV
Article Snippet: .. After blocking, the membrane was incubated overnight at 4°C with anti-SFTSV Gn antibody (1:1000 dilution, NBP2-41156, Novus Biological, Centennial, CO, USA) and anti-beta actin antibody (1:3000 dilution, 3700S, Cell Signaling Technology, Danvers, MA, USA). .. Anti-rabbit or anti-mouse antibody conjugated with horse radish peroxidase (1:5000 dilution, A120-101P or A90-116P, BETHYL, Montgomery, TX, USA) was used as the secondary antibody.
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